• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit the IL-6 R alpha enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. Saito, T.     et al. (1991) J. Immunol.     147:168. The ED    ₅₀ for this effect is 8-40 ng/mL in the presence of 10 ng/mL recombinant human (rh) IL-6 sR and 20 ng/mL rhIL-6.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse gp130 (Gln23 - Glu617) Accession # Q6PDI9	DIEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln23 predicted    
   

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  94 kDa (monomer)

• SDS-PAGE

  130 kDa, reducing conditions

Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

• References:

1. Narazaki, M. et al. (1993) Blood 82:1120.

2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

• Long Name:

  Glycoprotein 130

• Entrez Gene IDs:

  3572 (Human); 16195 (Mouse); 25205 (Rat)

• Alternate Names:

  CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha