• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit the IL-3-induced proliferation of NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED    ₅₀ for this effect is 0.2?1 μg/mL in the presence of 2.5 μg/mL soluble rmIL-3 R alpha /Fc Chimera and 0.5 ng/mL of rmIL-3..

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse IL-3 R beta (His23 - Trp440) Accession # Q3U3P4	IEGRID	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  His23

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  74.9 kDa (monomer)

• SDS-PAGE

  80 kDa, reducing conditions

Background: IL-3 R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha and the beta  subunits are members of the cytokine receptor superfamily (3).

• References:

1. Itoh, N. et al. (1990) Science 247:324.

2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.

3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.

• Long Name:

  Interleukin 3 Receptor beta

• Entrez Gene IDs:

  12984 (Mouse)

• Alternate Names:

  AIC2A; BetaIl3; Bil3; Csfgmrb; IL-3 R beta; IL3R beta; IL3R; IL3RB; IL-3Rb; Il3rb2