• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells.

  When 5 x 10⁴ cells/well are added to Recombinant Mouse L?Selectin/CD62L Fc Chimera coated plates, adhesion is induced in a dose dependent manner after a 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.3?1.2 μg/mL.

  Optimal dilutions should be determined by each laboratory for each application.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse L-Selectin (Trp39 - Asn332) Accession # Q3UV83	IEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Trp39

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  60.5 kDa (monomer)

• SDS-PAGE

  150 kDa, reducing conditions

Background: L-Selectin/CD62L

L-Selectin (also known as Leukocyte Selectin, LAM?1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, and CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90 - 100 kDa. Human and mouse L-Selectin share 76% amino acid sequence homology.

L-Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble L-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of L?Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

• Entrez Gene IDs:

  6402 (Human); 20343 (Mouse); 29259 (Rat)

• Alternate Names:

  CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1