• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.1 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Mouse OSM R beta  Fc Chimera is immobilized at 0.5 μg/mL and Recombinant Mouse gp130 Fc Chimera (Catalog # ) at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse Oncostatin M/OSM (Catalog # ) that produces 50% of the optimal binding response is approximately 0.2?1 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse OSM R beta (Glu24-Leu738) Accession # O70458	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

  
   

• Accession #

• N-terminal Sequence    
  Analysis

  Glu24

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  109 kDa (monomer)

• SDS-PAGE

  120-150 kDa, reducing conditions

Background: OSM R beta

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) residue type I transmembrane protein which contains a 23 aa residue signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity, but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.

• References:

1. Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.

2. Tanaka, M. et al. (1999) Blood 93:804.

• Long Name:

  Oncostatin M Receptor beta

• Entrez Gene IDs:

  9180 (Human); 18414 (Mouse); 310132 (Rat)

• Alternate Names:

  OSM R beta; OSMR; OSMRB