• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit Hep3B human hepatocellular carcinoma cell survival. The ED    ₅₀ for this effect is 2-10 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Arg21-Ala847, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Arg21

• Predicted Molecular Mass

  94 kDa

• SDS-PAGE

  85-98 kDa, reducing conditions    
   

Data Images

Background: Follistatin-like 5/FSTL5

Follistatin-like 5 (FSTL5) is a secreted 94 kDa (predicted) member of the SPARC protein family (1). In its N-terminal half, FSTL5 contains a Kazal-like domain, two EF-hand domains, and two Ig-like domains. Mature mouse FSTL5 shares 91% and 97% amino acid sequence identity with human and rat FSTL5, respectively. FSTL5 is expressed in the cartilage and tendon of developing digits and the shafts of long bones (2). In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum (3). FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells (4). Its expression in medulloblastoma and downregulation in hepatocellular carcinoma are associated with poor prognosis (4, 5).

• References:

1. Bradshaw, A.D. (2012) Int. J. Biochem. Cell Biol. 44:480.

2. Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.

3. Masuda, T. et al. (2014) Congenit. Anom. (Kyoto) 54:63.

4. Zhang, D. et al. (2015) Int. J. Clin. Exp. Pathol. 8:3386.

5. Remke, M. et al. (2011) J. Clin. Oncol. 29:3852.

• Entrez Gene IDs:

  56884 (Human); 213262 (Mouse); 365823 (Rat)

• Alternate Names:

  Follistatin like 5; Follistatin-like 5; Follistatin-like protein 5; follistatin-related protein 5; FSL5; FSTL5; KIAA1263DKFZp566D234