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Human/Mouse Draxin Affinity Purified Polyclonal Ab 25 UG

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Human/Mouse Draxin Affinity Purified Polyclonal Ab 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說(shuō)明

    • Species Reactivity

      Human, Mouse

    • Specificity

      Detects human Draxin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Draxin is observed.

    • Source

      Polyclonal Sheep IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      Mouse myeloma cell line NS0-derived recombinant human Draxin    
      aa 26-349    
      Accession # Q8NBI3

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1 μg/mL

      See below


    • Immunohistochemistry

      5-15 μg/mL

      See below


    • Immunocytochemistry

      5-15 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human Draxin by Western Blot. Western blot shows lysates of SH?SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 μg/mL of Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Immunocytochemistry      
         

    Draxin in SH?SY5Y Human Cell Line. Draxin was detected in immersion fixed SH?SY5Y human neuroblastoma cell line using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for .

    Immunohistochemistry      
         

    Draxin in Mouse Embryo. Draxin was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 15 μg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counter-stained with hematoxylin (blue). Specific staining was localized to diencephalon. View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Draxin

    Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58 kDa, presumable glycoprotein member of the draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature human Draxin is 324 amino acids (aa) in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa 274-333). Mature human Draxin (aa 26-349) shares 79% aa identity with mature mouse Draxin.

    • Long Name:

      Dorsal Repulsive Axon Guidance Protein

    • Entrez Gene IDs:

      374946 (Human); 70433 (Mouse); 691317 (Rat)

    • Alternate Names:

      AGPA3119; C1orf187; chromosome 1 open reading frame 187; dorsal inhibitory axon guidance protein; Dorsal repulsive axon guidance protein; Draxin; FLJ34999; MGC117222; Neucrin; UNQ3119



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