詳細(xì)說明
概述
產(chǎn)品名稱Anti-PPP2R5E抗體[EPR17147] - C-terminal
描述
兔單克隆抗體[EPR17147] to PPP2R5E - C-terminal
經(jīng)測試應(yīng)用WB,IP,ICC/IF,IHC-P
種屬反應(yīng)性
與反應(yīng): Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPP2R5E aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q16537陽性對照
WB: HeLa, 293 and Human skeletal muscle lysates. IHC: Human cervix carcinoma and bladder transitional cell carcinoma tissues. ICC/IF/IP: HeLa and MCF7 cells.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR17147
同種型IgG
研究領(lǐng)域
Signal Transduction
Protein Phosphorylation
Ser / Thr Phosphatases
Neuroscience
Neurotransmission
Intracellular Signaling
Phosphatases
Anti-PPP2R5E antibody [EPR17147] - C-terminal 圖像
![Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198500500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma) cells labeling PPP2R5E with ab198500 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor? 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor?594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab198500 at 1/500 dilution followed by ab150120 (AlexaFluor?594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor?488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1985000-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (Human breast carcinoma) cells labeling PPP2R5E with ab198500 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor? 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor?594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab198500 at 1/500 dilution followed by ab150120 (AlexaFluor?594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor?488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.Western blot - Anti-PPP2R5E antibody [EPR17147] (ab198500)
All lanes : Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500) at 1/5000 dilution
Lane 1 : HeLa cell lysate at 10 μg
Lane 2 : 293 cell lysate at 10 μg
Lane 3 : Human skeletal muscle lysate at 20 μg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
developed using the ECL technique
Predicted band size : 55 kDa
Observed band size : 55 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] (ab198500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19850000b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] (ab198500)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling PPP2R5E with ab198500 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cervix carcinoma tissue isobserved. Counter-stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] (ab198500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19850000c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] (ab198500)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PPP2R5E with ab198500 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue isobserved. Counter-stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody.
![Immunoprecipitation - Anti-PPP2R5E antibody [EPR17147] (ab198500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198500opy.jpg)
Immunoprecipitation - Anti-PPP2R5E antibody [EPR17147] (ab198500)
PPP2R5E was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma) whole cell extract with ab198500 at 1/175 dilution. Western blot was performed from the immunoprecipitate using ab198500 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell extract 10μg (Input).
Lane 2: HeLa whole cell extract
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198500 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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